Table 1 Primers used for RT-qPCR
From: β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275
Primer | Forward sequence (5′–3′) | Reverse sequence (5′–3′) |
|---|---|---|
miR-1275 | CGTGGGGGAGAGGCTGTC | Involved in the kit |
U6 | CTCGCTTCGGCAGCACATA | AACGATTCACGAATTTGCGT |
MIR143HG | AGAAGCAAGAACTCTGGAGA | TCTGCCTCTGTGTTCCCCATG |
ILK | ATGGAACCCTGAACAAACACT | AGCACATTTGGATGCGAGAAA |
Akt | GGACAACCGCCATCCAGACT | GCCAGGGACACCTCCATCTC |
Bcl-2 | TTCTTTGAGTTCGGTGGGGTC | TGCATATTTGTTTGGGGCAGG |
Cyclin D1 | GTGCATCTACACCGACAACTCCA | TGAGCTTGTTCACCAGGAGCA |
GAPDH | CGGAGTCAACGGATTTGGTCGTAT | AGCCTTCTCCATGGTGGTGAAGAC |