Fig. 1
Worenine reduces cell viability and impairs cell cycle progression. a The chemical structures of coptisine and worenine. b HCT116 and SW620 cells (1 × 104 cells per well) were treated with various concentrations of worenine (0, 1, 5, 10, 20, 40 or 80 μM) for 24 h. HCT116 and SW620 cells (1 × 104 cells per well) were treated with 20 μM worenine for 3, 6, 12, 18 or 24 h. Cell viability was detected using the MTT assay. c FHC cells were treated with worenine for 24 h at the indicated concentrations. d The colony formation rates of HCT116 cells treated with various concentrations of worenine (5, 10 or 20 μM) for 24 h. The percentage values are based on comparison with the control group. e Cell cycle profiles of HCT116 cells treated with various concentrations of worenine (5, 10 or 20 μM) for 24 h. The data are means ± SEM. #p < 0.05, ##p < 0.01 vs. control group