Fig. 2
MnCl2 induced mitophagy in SH-SY5Y cells. a, b BNIP3, LC3 and TOMM20 protein levels were measured by western blot after SH-SY5Y cells were treated with 200, 400, 800 µM MnCl2 (a) and treated with 400 µM MnCl2 alone or co-treated with mitophagy inhibitor 3-MA together (b) for 24 h. β-actin served as an internal reference. Compared with control, *P < 0.05, **P < 0.01. c Mitophagy bodies and mitochondria treated with 400 μM MnCl2 alone or co-treated with mitophagy inhibitor 3-MA together for 24 h were observed by transmission electron microscopy (TEM). N, nucleus. White arrow, expanded, condensed or dissolved, mitochondria aggregate. Black arrowhead (
), relatively normal mitochondrial morphology. Black arrow (
), mitophagy bodies