Fig. 5
BNIP3 mediated MnCl2-induced ROS generation and neurotoxicity. a, b, c After treatment with or without 400 µM MnCl2, the levels of ROS (a), the mitochondrial membrane potential (b), and cell apoptosis (c) were assessed in cells with or without BNIP3 shRNA lentivirus. NC, negative control treatment. NC + Mn, cells treated with 400 µM MnCl2 but not treated with lentivirus carrying interference sequences at 24 h. BNIP3-Ri, BNIP3 shRNA-transfected cells. BNIP3-Ri + Mn, cells treated with 400 µM MnCl2 and transfected with BNIP3 shRNA at 24 h