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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: MiR-501 promotes tumor proliferation and metastasis by targeting HOXD10 in endometrial cancer

Fig. 4

HOXD10 is a direct target of miR-501. A At the mRNA level, expression of HOXD10 in endometrial cancer tissues was lower than that in benign endometrial tissue. B The mRNA level of HOXD10 was lower in miR-501 up-regulated endometrial cancer cells than in the control. C Western blot analysis showed higher protein level of HOXD10 in benign endometrium samples than endometrial cancer tissues (N1–N9 present benign endometrium samples, TI–T16 were collected from endometrial cancer patients). D Western blot analysis showed that expression of HOXD10 had an inverse relationship with miR-501 in endometrial cancer cells. E Expression of HOXD10 detected by immunohistochemistry. (i) Representative micrograph showing expression of HOXD10 in endometrial cancer tissues and control; (ii) Expression of HOXD10 was higher in benign endometrium than endometrial cancer tissues. F Putative binding sequence of miR-501 in the HOXD10 3’UTR, the binding sequence of miR-501 in the HOXD10 3’UTR sequence was deleted to construct the mutant. G Overexpression of miR-501 could reduce the luciferase activity when the luciferase reporter plasmid contained WT HOXD10 3’UTR, but no difference was found in the mutant (MT) type. **p < 0.01; ****p < 0.0001

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