Fig. 2
Expression and transcriptional regulation of miR-127-5p during chondrogenic differentiation of rMSCs under hypoxic conditions. The rMSCs were induced to undergo chondrogenic differentiation under hypoxic conditions (1% O2) for 7 and 14 days. A Expression of COL1A1, COL2A1, SOX9, and ACAN proteins in rMSCs in the control, induction, hypoxia, and hypoxia/induction groups was detected by western blotting. B MiR-127-5p expression was determined by quantitative real-time PCR analysis. C Transcriptional factors (ERRγ and SHP) were analyzed by western blotting. D SHP protein expression was detected by immunofluorescence. rMSCs were transfected with si-SHP, followed by hypoxia intervention for 24 and 48 h. E Western blotting was performed to examine the relative levels of HIF-1α and SHP protein expression in rMSCs from the control, hypoxia, and si-SHP/hypoxia groups. Quantitative real-time PCR was used to examine SHP (F) and miR-127-5p expression (G). Results represent the mean value ± standard deviation of data obtained from three independent experiments. ***p < 0.001, compared with control; ###p < 0.001, compared with hypoxia