Fig. 1
From: Macrophage polarization by MSC-derived CXCL12 determines tumor growth
Cell characteristics of bone marrow derived macrophages (BMDMs) and mesenchymal stem cells (MSCs). Phase contrast microscopic image of BMDMs 5 days after in vitro culture (A). Flow cytometric analysis of BMDMs for F4/80 (B). Flow cytometric analysis of MSCs for CD90, CD105, CD34, and CD45 (C). CXCL12 gene knockout in MSCs. Transfection of MSCs with a GFP-positive CRISPR/Cas9 plasmid to knock out CXCL12 gene (D). Western blotting for knockout efficiency (E). β-actin was used as a control. CXCL12 measurement in the serum-free conditioned media of MSCCXCL12+/+ and MSCCXCL12-/- by ELISA (F). Each point indicates an experiment (horizontal lines: mean)