Fig. 2
From: Macrophage polarization by MSC-derived CXCL12 determines tumor growth
Macrophage phenotypic switching by MSC-derived CXCL12 niche. Schematic diagram of macrophage and MSC cocultures. Bone marrow macrophages were cocultured with MSCs in non-contact Transwell inserts for 48 h. Then, macrophages were incubated for a further 24 h before submission for phenotype and cytokine assays (A). Cytokine levels of IL10, IL4, TGF-β, IL6 and TNF-α in supernatants of macrophages after non-contact coculture with MSCsCXCL12+/+ (M2) or MSCsCXCL12−/− (M1). Each point indicates an experiment (horizontal lines: mean) (B). Western blot depicting protein levels of iNOS (C). Left and right lanes indicate iNOS in Mac in the presence of MSCsCXCL12+/+ and MSCsCXCL12−/−, respectively. Representative data from three independent experiments (C). Quantitative analysis of iNOS levels (D). Representative flow cytometry dot plots of CD206 expression by macrophages after culturing with MSCsCXCL12+/+ (M2) or MSCsCXCL12−/− (M1). MSC-derived CXCL12 niche strongly induced CD206 expression by macrophages (E). Representative FSC/SSC and F4/80 gating is shown. Data from three independent experiments with the mean percentage of positive cells in each group are shown within histograms (E). *P < 0.5, ***P < 0.001, ****P < 0.0001, Student's t-test. Mac, macrophage