Fig. 5
From: Macrophage polarization by MSC-derived CXCL12 determines tumor growth
Multicellular tumor spheroids (MCTS) by 4T1 cells after 72 h coculture with MSCCXCL12−/−-primed (A) and MSCCXCL12+/+-primed macrophages (B). 4T1 tumor cells and macrophages were labeled with CM-DiI and CFSE, then cocultured in the Vitrogel matrix. Representative mask images of MCTS by 4T1 cells in each group produced by the MATLAB software (C). The number of 4T1 colonies and the size of tumor spheroids were greater after coculturing 4T1 cells with macrophages primed with MSCsCXCL12+/+ (D). *P < 0.5, Student's t-test. Mac, macrophage