Fig. 3

T-MSC differentiation into neuronal stem cells (NSCs). qRT-PCR from neurospheres (day-3) from 5 different donors and BM-MSCs show an increase in neural stem cell markers A MSI1, B Nestin, and C SOX2 compared to undifferentiated isogenic controls. Results are presented as mean ± S.E.M. obtained in triplicates; n = 5 donors in multiple independent experiments. Asterisks signify **p < 0.01, ***P < 0.001, ****p < 0.0001. D immunofluorescence analysis for neuronal stem cell markers in T-MSCs differentiated for 4 days to T-NSCs versus BM-NSCs in similar conditions: Nestin (green) co-stained with MSI1 (red). E SOX1 (red) and Nestin (green). Nuclei are stained with DAPI (blue). Undifferentiated T-MSCs and BM-MSCs were used as additional controls (see Additional file 1: Figure S1)