Fig. 5
From: Direct differentiation of tonsillar biopsy-derived stem cells to the neuronal lineage
T-MSC acquire neuron-like phenotype following 28 days of differentiation. qRT-PCR results show a continued upregulation of the neuronal markers: A DCX, B Tuj1, C MAP2, D NEFL, and E PSD95 after 28 days of differentiation. 2 representative donor lines are shown. Result presented as mean ± S.E.M. Results were validated in n = 5 donors in multiple independent experiments. Data shown as mean ± SEM. Asterisks signify *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. F Consistently, co-immunofluorescence staining shows a similar upregulation of neuronal markers MAP2, Pan-neurofilament, neurofilament-L (NEFL) and G Synaptophysin (SYP) DAPI serves as a nuclear counterstain