Fig. 7

Loss of proliferation and the MSC phenotype with neurogenic differentiation. Quantification of immunostaining analyses averaged from multiple donors indicate a dramatic reduction in the replication markers A KI67 and B Cyclin A2. Asterisks signify **p < 0.01, ****p < 0.0001; n = 5 in triplicates. C qRT-PCR results show a major decrease of the pericyte marker ACTA2 and D the MSC marker CD90 after 72 h of differentiation as neurospheres. Data shown as mean ± SEM. Asterisks signify ***p < 0.001, ****p < 0.0001. Data from 3 representative donors are shown. Consistently, flow cytometry analyses of MSC markers in day 11 neuroblasts, demonstrate both low intensity and an overall decrease in positive cells for the MSC markers: E CD73 with 16.4%; F CD90 with 4.2% and G CD105 6.4%. H Flow cytometry further show that the vast majority of the cells no longer co-express the MSC markers CD90 and CD105, I CD73 and CD105 and J CD73 and CD90. Data show representative experiments from 3 different donors in biological duplicates