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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Apoptosis-promoting properties of miR-3074-5p in MC3T3-E1 cells under iron overload conditions

Fig. 3

miR-3074-5p mediated the apoptosis of MC3T3-E1 cells under iron overload conditions. A Gain- and loss-of-function experiments were performed by transfecting cells with a miR-3074-5p mimic and miR-3074-5p inhibitor, respectively. The CCK-8 assay was used to investigate the effect of miR-3074-5p on cell viability. *P < 0.05 vs. the NC. #P < 0.05 vs. the NC + FAC group. ns P > 0.05 vs. the NC group. FAC concentration: 1.8 mM. B Annexin V-FITC/PI staining was performed to confirm the function of miR-3074-5p in cell apoptosis. C Quantitative analysis of late apoptosis rate based on Annexin V-FITC/PI staining. *P < 0.05 vs. the NC. #P < 0.05 vs. the NC + FAC group. ns P > 0.05 vs. the NC group

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