Table 2 Primer sequences for qRT-PCR
From: Apoptosis-promoting properties of miR-3074-5p in MC3T3-E1 cells under iron overload conditions
Gene | Sequences |
|---|---|
miR-3074-5p | Forward: 5′-GCGGTTCCTGCTGAACTGA-3′ |
Reverse: 5′-AGTGCAGGGTCCGAGGTATT-3′ | |
Smad4 | Forward: 5′-AGTTCACAATGAGCTTGCATTC-3′ |
Reverse: 5′-TTCAAAGTAAGCAATGGAGCAC-3′ | |
U6 | Forward: 5′-TGCAGAGGATCTAATT-3′ |
Reverse: 5′-GAAAGACCAGTCCAAGTCC-3′ | |
GAPDH | Forward: 5′-TGTGTCCGTCGATCTGA-3′ |
Reverse: 5′-TTGCTGTTTGTTGAAGTCGCAGGAG-3′ |