Fig. 5From: Comprehensive RNA sequencing in primary murine keratinocytes and fibroblasts identifies novel biomarkers and provides potential therapeutic targets for skin-related diseasesValidation of skin disease related genes in RNA-Seq data using qRT-PCR. Seven skin disease related genes were selected to be analyzed by qRT-PCR. The relative mRNA expression was expressed as 2−ΔΔCT. The Gapdh gene served as an internal reference to normalize the target gene expression. Mean ± SD (n = 3). *P < 0.05, **P < 0.01Back to article page