From: Targeting microRNAs with thymoquinone: a new approach for cancer therapy
Cancer type | Cell lines | Animal model | TQ dosage | miRNA type | Effect on miRNA | Mechanism of action of thymoquinone | References |
---|---|---|---|---|---|---|---|
Pancreatic cancer | PANC-1 MIA PaCa-2 | – | In vitro: 6.25 μM | miR-24–1 miR-101 | Up-regulation | Cleavage of procaspase3, PARP, increased PKM2 expression | [105] |
Breast cancer | MDA-MB-231 MDA-MB-468 | Mouse | In vivo: 3 μg/mL | miR-361 | Up-regulation | Down-regulated Rac1, RhoA and VEGF-A, inhibit both metastasis and angiogenesis | [106] |
Lung cancer | - | Mouse | In vivo: 10Â mg/kg | miR-206 | Down-regulation | Reduction of oxidative stress and necrosis formation, regeneration of the liver tissue | [110] |
Breast cancer | MCF-7 cells | Mouse | Â | miR-34a | Up-regulation | Up-regulation of p53, down-regulation of Rac1, Metastasis inhibition | [25] |
Breast cancer | BT-549 | – | In vitro: 5 μM | miR-34a | Up-regulation | Decreased levels of EMT-TFs including Twist and Snail, ZEB and NOTCH, control metastasis through down-regulation of EMT | [83] |
Leukemia | MV4-11 and Kasumi-1 | Mouse | In vitro: 10 μM In vivo: 15 mg/kg | miR-29b | Up-regulation | Dysfunction of DNA methyltransferases, dissociation of Sp1/NF-κB complex, induces apoptosis through activation of caspase-3 and caspase-8 | [66] |
Breast cancer | MDA-MB-231 MDA-MB-436 | Mouse | In vitro: 15 μM In vivo: 100 mg/kg | miR-34a | Up-regulation | Suppressing NF-κB and eEF-2 K pathway | [65] |
Liver cancer | HepG2, Huh7 | – | In vitro: 10 μM | miR-16 and miR-375 | Up-regulation | Up-regulated caspase-3, down-regulated Bcl-2 | [114] |
Lung cancer | A549 | Mouse | In vitro: 5 μM In vivo: 5 mg/kg | miR-16 and miR-375 | Up-regulation | Up-regulation of p53 and Bax, down-regulation of Bcl2, pro-caspase-3 and pro-caspase-9, induce apoptosis | [26] |