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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Primary high-grade serous ovarian cancer cells are sensitive to senescence induced by carboplatin and paclitaxel in vitro

Fig. 1

Development of the senescence phenotype in HGSOCs treated with CPT-PCT. For details regarding the procedure of CPT-PCT-dependent senescence induction please refer to “Methods”. Quantification of the fraction of cells displaying biochemical (SA-β-Gal) and molecular (γ-H2A.X, and 53 BP1) markers of cellular senescence in young and DIPS cancer cells (a). Representative staining against senescence biomarkers. SA-β-Gal-positive cells are characterized by green cytoplasm. White arrows indicate exemplary γ-H2A.X- and 53BP1- positive cells (b). Representative illustrations of young and DIPS cells’ appearance obtained upon changing the color image to black and white, sharpening and increasing contrast in cells stained for SA-β-Gal (c). Magnification × 200, objective aperture 0.35. The results are based on 6–8 independent experiments using HGSOCs obtained from different patients. The results are expressed as the means ± SEM. **p < 0.01; ***p < 0.001 vs. young cells

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