Fig. 5

MYCBP overexpression counteracted the suppressive effect of miR-26b-5p on TNBC cell behaviors. MDA-MB-468 and BT-549 cells were co-transfected with MYCBP overexpressing plasmid and miR-26b-5p mimics. The expression levels of MYCBP mRNA (A) and protein (B) in MDA-MB-468 and BT-549 cells after indicated transfection were determined by PCR and western blot assays. C Cell viability was determined using CCK-8 assay in MDA-MB-468 and BT-549 cells after indicated transfection. D The number of colonies in MDA-MB-468 and BT-549 cells after indicated transfection. Cell migration (E) and invasion (F) were assessed by transwell assay in MDA-MB-468 and BT-549 cells after indicated transfection. Magnification, ×200; scale bar, 100 μm; data are shown as mean ± SD (n = 3). ***p < 0.001 compared with miR-NC + Vector group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with MYCBP or miR-26b-5p mimics; G The protein levels of E-cadherin, N-cadherin and vimentin were measured by western blot analysis in MDA-MB-468 and BT-549 cells after indicated transfection