Fig. 4

KMT2D increases LIFR and KLF4 expression in an LCD-dependent manner. a Representative images of cell immunofluorescence determining the H3K4me1 expression in PANC-1 cells. After LCD knockout, the H3K4me1 level was markedly reduced in the nucleus, but transfection of full-length KMT2D plasmids could reverse the decrease in H3K4me1 expression. b, c Western blotting analyses of the H3K4me1, LIFR, and KLF4 proteins in HEK293T and PANC-1 cells. Knockout of KMT2D LCD significantly attenuated the H3K4me1 expression and decreased LIFR and KLF4 protein expression. LIPR, 190 KDa; KLF4, 62 KDa; H3K4me1, 17 KDa; H3 (histone H3), 17 KDa. d, e RT-PCR analyses of LIFR and KLF4 expression. After LCD deletion in HEK293T and PANC-1 cells, the gene expression of LIFR and KLF4 decreased compared to the control group (vehicle; error bars denote standard deviation). All values are represented as means ± SD. Data are representative of three independent experiments