Fig. 4

ESD stabilized cytoplasmic FKBP25. a, b Western blot analysis of FKBP25 and ACTB level in A549 cells treated with FPD5 at 0–5 μM for 24 h. c, d Western blot analysis of FKBP25 level in HEK293T-WT and HEK293T-ESD^−/−. e, f HEK293T cell line was transfected with plasmids pCAG-ESD-WT and pCAG-ESD-K213A for 48 h. Then the level of FKBP25 was analyzed by western blot. g, h Western blot analysis of FKBP25 and ACTB level in A549 cells treated with FPD5 at 5 μM for 24 h in the presence of proteasome inhibitor MG132. i–k Western blot analysis of FKBP25, GAPDH and lamin A/C level in cytoplasm and nucleus of A549 cells treated with FPD5 at 0–5 μM for 24 h. Data are mean ± SEM. *p < 0.05, **p < 0.01, N.S., not significant, n = 3