Fig. 2

HCG18 enhanced aerobic glycolysis in OS cells. A and B ECAR of sh-Control and sh-HCG18 group was detected. Glc: glucose, Oligo: oligomycin, 2-DG: 2-deoxy-d-glucose. C and D OCR of sh-Control and sh-HCG18 group was measured. O: Oligomycin, F: FCCP, A&R: antimycin A/rotenone. Glucose consumption (E), lactate production (F), and ATP level (G) demonstrated the effect of HCG18 knockdown on aerobic glycolysis in OS cells. ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated three times