Fig. 3

PGK1 was the target of HCG18. A PGK1 expression in OS cell lines and in hFOB1.19. B Overexpression efficacy of PGK1 in sh-HCG18 OS cells. CCK8 (C and D), colony formation (E), EDU (F), and apoptosis (G and H) assays confirmed that overexpression of PGK1 partly reversed the suppressive effects of HCG18 knockdown on OS growth. I-L, ECR and OCR in OS cells in different groups (sh-Control, sh-HCG18, and sh-HCG18 + ov-PGK1) were measured. * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated three times