Fig. 4

MiR-365a-3p targeted PGK1 directly in OS cells. A Venn diagram showing the predicted target genes of PGK1 and HCG18 from databases (miRDB, TargetScan, and StarBase). B and C PGK1 expression in miRNA mimics the treated or Control MNNG-HOS and 143B cells. D PGK1 expression was measured by western blot in OS cells transfected with miR-365a-3p mimics or negative control. E Putative binding sites between PGK1 and miR-365a-3p. F and G Dual-luciferase reporter assay in OS cells transfected with miR-365a-3p mimics or negative control. ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated thrice