Fig. 5

HCG18 acted as a molecular sponge of miR-365a-3p. A Putative binding sites between HCG18 and miR-365a-3p. B and C Luciferase activity of OS cells in luciferase reporter plasmid containing wild-type HCG18 3ʹ-UTR and mutant HCG18 3ʹ-UTR co-transfected with miR-365a-3p mimics or negative control was assessed. D and E, AGO2-RIP followed by qPCR to detect HCG18 level after miR-365a-3p overexpression. F, Expression of miR-365a-3p was upregulated in OS cells transfected with HCG18 shRNA or the control shRNA by RT-qPCR. * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated three times