Fig. 6

MiR-365a-3p inhibition reversed the inhibitory effect induced by knockdown of HCG18 in OS cells. A Western blot showed the PGK1 expression of OS cells transfected with miR-365a-3p inhibitor, sh-HCG18, or negative control. Colony formation (B), and apoptosis (C and D) assays confirmed that knockdown of miR-365a-3p partly reversed the suppressive effects of HCG18 knockdown on OS proliferation. Knockdown of miR-365a-3p in wild-type OS cells (MNNG-HOS and 143B) also improved OS proliferation. E and F, Altered levels of lactate concentration and generative ATP in different groups (sh-Control, sh-HCG18, sh-HCG18 + anti- miR-365a-3p and anti- miR-365a-3p) was measured. * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001. All experiments were repeated three times