Table 2 The sequences of specific primers
Gene name | Primer sequence (5ʹ to 3ʹ) |
|---|---|
DANCR | Forward: 5ʹ-GCCACTATGTAGCGGGTTTC-3ʹ Reverse: 5ʹ-ACCTGCGCTAAGAACTGAGG-3ʹ |
miR-214-5p | Forward: 5ʹ-GCCGAGTGCCTGTCTACACT-3ʹ |
Reverse: 5ʹ-CTCAACTGGTGTCGTGGA-3ʹ | |
COX20 | Forward: 5ʹ-TCTGTTGTGGCTGGCTTTGGAC-3ʹ Reverse: 5ʹ-CTTCTCTGGCAATTCTTTCCTGG-3ʹ |
GAPDH | Forward: 5ʹ-ATCCACGGGAGAGCGACAT-3ʹ Reverse: 5ʹ-CAGCTGCTTGTAAAGTGGAC-3ʹ |
U6 | Forward: 5ʹ-ACAGATCTGTCGGTGTGGCAC-3ʹ Reverse: 5ʹ-GGCCCCGGATTATCCGACATTC-3ʹ |