Fig. 2

PADI2 overexpression increases apoptosis in Jurkat T cells. A The colorimetric method was used to determine the activity of the PADI2 enzyme [60]. The data are presented as the mean ± SEM of three independent experiments (***p < 0.001). The p value in was determined by one-way ANOVA. B Cell viability of Tet-On-Vector and Tet-On-PADI2 cells without or with 50 μM Dox treatment was determined by trypan blue exclusion. The data are presented as the mean ± SEM of three independent experiments (***p < 0.001). The p value in was determined by one-way ANOVA. C The bar graphs show the percentages of cells with apoptotic bodies with 50 μM Dox treatment for 48 h. The data are presented as the mean ± SEM of three separate experiments (***p < 0.001). The p value in was calculated by Student’s t-test. D Antibodies against PADI2, Bcl-xL, Bax, cleaved-caspase 3, cleaved-PARP, and β-actin were used to detect proteins extracted from Tet-On-Vector and Tet-On-PADI2 cells 48 h after exposure to 50 μM Dox. The value indicates the ratio of protein signal to β-actin signal