Fig. 7

PADI2 overexpression results in the activation of T helper 17-like T cells and the production of pro-inflammatory cytokines. The Tet-On-Vector or Tet-On-PADI2 Jurkat T cells were treated with 50 µM Dox for 12 h. A The mRNA expression levels of the transcription factors STAT3, RORγt, C/EBP-β, T-bet, GATA3, and Foxp3 and β-actin were determined using RT-PCR. B RT-PCR was used to determine the levels of IL-17A, IL-22, IL-21, IL-6, and β-actin mRNA expression at 0, 12 h, and 24 h. C Transfection of cells with a luciferase vector containing the IL-4 promoter, the IL-17 promoter, or the STAT3 binding-site promoter was performed. After 12 h of treatment with 50 µM Dox, the luciferase activity was determined. The data are presented as the mean ± SEM of three separate experiments (**p < 0.01, and ***p < 0.001). The p value in was calculated by two-way ANOVA. D Following 4-PBA treatment, the mRNA expression levels of STAT3, IL-6, IL-17A, IL-22 and β-actin were determined. The value indicates the ratio of protein signal to β-actin signal