Fig.5

PRPF3 promotes pancreatic cancer proliferation, migration, and invasion in vitro via the RAP2B/ERK pathway. A, B Cell counting assays showed the effect of PRPF3 on pancreatic cancer cells proliferation in MIAPaCa-2 A and SW1990 B cells. C, D Colony formation assays were performed in MIAPaCa-2 (C) and SW1990 D cells. E, F The effect of PRPF3 on the cell cycle in MIAPaCa-2 (E) and SW1990 F cells were detected. G, H transwell assays were performed to detect the migration and invasion abilities of PRPF3-silenced MIAPaCa-2, and SW1990 cells and control cells. I The indicated protein expression levels in PRPF3-knockdown MIAPaCa-2, SW1990 cells, and control cells were detected. J Western blot results showing the indicated protein expression levels in SW1990 cells stably transfected with control, shPRPF3, and shPRPF3 + RAP2B. K–M Cell counting, colony formation, and transwell assays showing that the suppression of proliferation, migration, and invasion by shPRPF3 in SW1990 cells was partially abolished by RAP2B. Scale bar = 100 μm. Results are shown as the mean ± SD of three independent replicates. *p < 0.05, **p < 0.01, ***p < 0.001