Fig. 8

Validation of ROBO1 as a gene target of miR-152-3p. miR-152-3p binds with the 3′-UTR of ROBO1 (A). In vitro luciferase activity in HepG2 and Huh7 cells (B). ROBO1 protein expression in HepG2 and Huh7 cells with miR-152-3p mimics transfection (C). Cell proliferation (D) and apoptosis (E) of HepG2 and Huh7 cells were analysed using CCK8 and TUNEL. ^*p < 0.05 versus miR-Con group; ^#p < 0.05 versus sh-Con group; ^$p < 0.05 versus mimics group