Fig. 1

shRNA depletion of p63 reduces proliferation. A ΔNp63 western blotting in HaCaT or FaDu cells containing doxycycline-inducible p63 shRNAs with or without doxycycline (DOX) for 24 h. β-actin is shown as loading control. Relative densitometry measurements of ΔNp63 are shown after normalizing to β-actin; control cells are designated as 1 for each clone. B Crystal violet staining of colonies formed with or without doxycycline for 4 days from 2 stable clones of HaCaT or FaDu shRNA-p63 cells. C Percentages of Ki67 in HaCaT or FaDu cells growing with doxycycline (DOX) or control cells without doxycycline (Con) for 4 days. *p < 0.05;***p < 0.001 for doxycycline treated cells compared to the same cells growing without doxycycline