Fig. 3From: Identifying pathways regulating the oncogenic p53 family member ΔNp63 provides therapeutic avenues for squamous cell carcinomaThe effects of growth factor signaling pathways. Representative western blots of ΔNp63 (72 kDa) in HaCaT, FaDu or SCC-25 cells grown for 16 h in 1% FBS and collected after a further 24 h of treatment with A the indicated growth factors (IGF1, insulin like growth factor-1; INS, insulin; EGF, epidermal growth factor; A, amphiregulin), B Cetuximab (CETUX), C BEZ235 (BEZ). β-actin (42 kDa) is shown as the loading control. CTR represents growth in standard medium (10% FBS); 0 represents growth in 1% FBS with no addition of growth factor or inhibitor. Relative ΔNp63 densitometry measurements normalized to β-actin are indicated for the representative blots shown, with 0 used as control. See Additional file 6 for other growth factors and Additional file 7 for the effects of inhibitors on downstream signaling pathwaysBack to article page