Fig. 4
Lovastatin selectively induces ΔNp63 protein and mRNA in HaCaT cells. Top panels; Representative western blots of ΔNp63 (72 kDa) and β-actin (42 kDa) as loading control for HaCaT, FaDu and SCC-25 cells treated for 24 h with the indicated concentrations of lovastatin (Lov). Relative ΔNp63 densitometry measurements normalized to β-actin are indicated, with 0 used as control. Lower panels; RT-qPCR for ΔNp63 mRNA shown as fold change after normalization to ACTB mRNA, with 0 used as control. *p < 0.05; **p < 0.01 compared to untreated cells