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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages

Fig. 5

IL10-MSCs induced M2/M1 polarization of BV2 cells upon LPS and IFN-γ challenges. A, D Immunofluorescence staining was performed to assess Arg1 and iNOS expression in BV2 upon LPS and IFN-γ challenges in the presence of MSC- or IL10-MSC-derived CM. B, C IL10-MSC-derived CM obviously increased the mRNA expression of M2 markers (Arg1 and IL10). E–H IL10-MSC-derived CM significantly decreased the mRNA expression of M1 markers (iNOS, TNF-α, IL1β, and IL-6) by qPCR. I–K FCA analysis was perform to investigate the percentage of M1 (CD11b+/F4/80+/iNOS+) and M2 (CD11b+/F4/80+/Arg1+) BV2 cells. (n = 6–8 per group, *p < 0.05, **p < 0.01, and ***p < 0.001)

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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