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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages

Fig. 6

The grafting of IL10-MSCs reduced the inhibition of neuronal differentiation and death of NSCs induced by activated BV2 cells. A Quantification of Tuj-1 staining revealed a significant increase in Tuj-1-positive cells in IL10-MSC group compared with vehicle (***p < 0.001) and MSC groups (**p < 0.01) via optical density analysis. B, C The rate of death and neural differentiation of NSCs after being co-cultured with IL10-MSCs and MSCs in the presence of BV2-derived CM. Live and dead cells were stained green and red, respectively. The percentage of neurons derived from NSCs was quantified by Tuj-1 immunofluorescent staining. Results revealed that IL10-MSCs could rescue the neuronal differentiation inhibition of NSCs induced by activated BV2 cells. Scale bars, 250 and 50 μm. Data are expressed as mean ± SEM (n = 6), *p < 0.05, **p < 0.01, ***p < 0.001

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