Fig. 2

ROR2 increased MDM2 and decreased p53 protein levels. ROR2 regulates p53 A and MDM2 B levels. Protein extracts from the indicated cell lines were probed with the indicated antibodies. GAPDH was used as loading control. The blots displayed are representative of three independent experiments. Bar graphs show the mean ± SD (from three independent experiments) of p53 A or MDM2 B levels normalized to the loading control and expressed as the fold change relative to control cells. Statistical significance was tested by a one-tailed Student’s t-test, ***p < 0.0001, n = 3. C, D Assessment of MDM2 localization by immunofluorescence upon ROR2 overexpression (C) or silencing (D). A375-Empty and A375-ROR2 (C) or M2-scramble and M2-shROR2 (D) were stained with DAPI and anti-MDM2 antibody