Fig. 2

ALKBH5 regulates processing of miR-320a-3p by DGCR8 in an m⁶A-dependent manner to have an antifibrotic effect. A Expression of ALKBH5, miR-320a-3p, and pri-miR-320a-3p in MRC-5 cells after transfection with siNC or siALKBH5 by qRT-PCR analysis with U6 as internal reference. Detection of miR-320a-3p (B) and pri-miR-320a-3p (C) m⁶A modification levels by RIP of m⁶A-modified miRNA in siNC or siALKBH5 groups followed by qRT-PCR. D Detection of pri-miR-320a-3p binding to DGCR8 by immunoprecipitation of DGCR8-associated RNA from MRC-5 cells with transfected siNC or siALKBH5 followed by qRT-PCR. E After co-transfection with siALKBH5 and miR-320a-3p inhibitor, MRC-5 cells were administrated 5 ng/ml TGF-β1 for 48 h. Western blotting showed fibronectin, collagen I, FOXM1, and α-SMA protein levels. All data expressed as mean ± SD of at least three independent experiments, *p < 0.05 and **p < 0.01