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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: ALKBH5 promotes lung fibroblast activation and silica-induced pulmonary fibrosis through miR-320a-3p and FOXM1

Fig. 6

FOXM1 is also regulated by ALKBH5 directly in an m6A-dependent manner. A Knockdown of ALKBH5 inhibited both FOXM1 mRNA and pre-mRNA expression, as showed by qRT-PCR analysis. B Western blot analysis showed that inhibition of ALKBH5 decreased FOXM1 protein expression. C and D Detection of FOXM1 mRNA and FOXM1 pre-mRNA m6A modification levels by immunoprecipitation of m6A-modified miRNA in siNC or siALKBH5 groups followed by qRT-PCR. E mRNA level of FOXM1 analyzed by qRT-PCR in actinomycin D-treated MRC-5 cells with transfected siNC or siALKBH5. All data expressed as mean ± SD of at least three independent experiments, *p < 0.05 and **p < 0.01

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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