Fig. 2

Knockdown of circRbms1 alleviated hypoxia-induced H9c2 cell injury. A, B qRT-PCR was used to assess circRbms1 expression to evaluate the transfection efficiency of si-circRbms1 (50 nM) or circRbms1 overexpression vector (4.0 µg) in H9c2 cells. C–G H9c2 cells were transfected with or without si-NC (50 nM), si-circRbms1 (50 nM), vector (4.0 µg) or circRbms1 (4.0 µg), and then treated with hypoxia. Untreated H9c2 cells were used as control. CCK8 assay (C), transwell assay (D, E) and flow cytometry (F) were used to determine cell viability, migrated and invaded cell numbers, and cell apoptosis rate, respectively. G WB analysis was performed to test the protein levels of Bcl-2, Bax, and Cleaved-caspase 3. All experiments were repeated three times. **P < 0.01, ***P < 0.001