Fig. 5

Effects of circRbms1 silencing and miR-742-3p inhibitor on hypoxia-induced H9c2 cell injury. A After transfecting with anti-NC (50 nM) or anti-miR-742-3p (50 nM) into H9c2 cells, the expression of miR-742-3p was assessed by qRT-PCR. B–F H9c2 cells were transfected with si-NC (50 nM), si-circRbms1 (50 nM), si-circRbms1 (50 nM) + anti-NC (50 nM), or si-circRbms1 (50 nM) + anti-miR-742-3p (50 nM), and then treated with hypoxia. Untreated H9c2 cells were used as control. CCK8 assay (B), transwell assay (C, D), and flow cytometry (E) were employed to examine cell viability, migrated and invaded cell numbers, and cell apoptosis rate, respectively. F WB analysis was used to test the protein levels of Bcl-2, Bax, and Cleaved-caspase 3. All experiments were repeated three times. **P < 0.01, ***P < 0.001