Fig. 6

FOXO1 was targeted by miR-742-3p. A The sequences of FOXO1-3′UTR-WT and FOXO1-3′UTR-MUT. B Dual-luciferase reporter assay was used to confirm the interaction between miR-742-3p and FOXO1. C, D H9c2 cells were transfected with miR-NC (50 nM), miR-742-3p (50 nM), anti-NC (50 nM), or anti-miR-742-3p (50 nM). The mRNA and protein expression levels of FOXO1 were measured using qRT-PCR and WB analysis. E, F H9c2 cells were transfected with si-NC (50 nM), si-circRbms1 (50 nM), si-circRbms1 (50 nM) + anti-NC (50 nM), or si-circRbms1 (50 nM) + anti-miR-742-3p (50 nM), and then treated with hypoxia. Untreated H9c2 cells were used as control. qRT-PCR and WB analysis were used to determine the mRNA and protein expression levels of FOXO1. G–H The mRNA and protein expression levels of FOXO1 in the heart tissues of sham mice and MI mice were examined using qRT-PCR (n = 6 per group) and WB analysis (n = 3 per group). All experiments were repeated three times. **P < 0.01, ***P < 0.001