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Fig. 10 | Cellular & Molecular Biology Letters

Fig. 10

From: Identification and validation of an eight-lncRNA signature that predicts prognosis in patients with esophageal squamous cell carcinoma

Fig. 10

Knockdown of LINC00470 suppresses ESCC cell proliferation, G1/S transition, and migration in vitro. A Transfection with si-LINC00470 dramatically suppressed LINC00470 expression in EC9706 and TE-9 cells. B CCK-8 assay showed that knockdown of LINC00470 resulted in growth retardation of EC9706 and TE-9 cells. Flow cytometry assay was conducted to analyze cell cycle distribution in transfected EC9706 C and TE-9 D cells. E Cell migration was evaluated in transfected EC9706 and TE-9 cells by transwell assay. Magnification, ×200; scale bar, 100 μm. F Western blot analysis was performed to determine the protein levels of PCNA, CDK4, E-cadherin, and N-cadherin in EC9706 and TE-9 cells. Data are expressed as mean ± SD. **p < 0.01, ***p < 0.001, compared with si-NC

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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