Skip to main content
Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Reversely immortalized mouse salivary gland cells presented a promising metabolic and fibrotic response upon BMP9/Gdf2 stimulation

Fig. 4

FLP recombinase-mediated removal of SV40 T antigen decreases proliferative activity of iSMGCs and iSLGCs. A FLP recombinase-mediated removal of SV40 T antigen in iSMGCs or iSLGCs. Cells were infected with Ad-FLP or Ad-GFP as the control. Quantity and intensity of green fluorescent cells was high at 24 h during these treatments. The growth status of Ad-FLP-transduced cells or Ad-GFP-treated cells is shown at passage 2 (p2). B SV40 T-antigen removal efficiency and the effects of multiplication capacity in cells. RT-qPCR confirmed that Ad-FLP decreased SV40 T-antigen expression significantly compared with Ad-GFP treatment. Meanwhile, the multiplication capacity of cells declined and was accompanied by the removal of the SV40 T antigen. Each assay condition was analyzed using a minimum of three independent experiments. *P < 0.05, **P < 0.01

Back to article page