Fig. 5

Differentially expressed gene analysis (DEG) and KEGG enrichment by RNA-seq. A Correlation heatmap showing correlation between SLG and SMG on E16.5 and P0. B In total, 608 upregulated (red dots) and 812 downregulated (green dots) DEGs with P < 0.001 were counted between SLG and SMG. C The top 20 KEGG pathways between SLG and SMG on P0 are listed. D RNA-seq analysis revealed upregulated (376) and downregulated (359) DEGs in iSMGCs, compared with in iSLGCs. E KEGG enrichment included eight signaling pathways based on the DEGs between iSMGCs and iSLGCs: 1–8 represent cytokine–cytokine receptor interaction, Jak–STAT signaling pathway, leishmaniasis, legionellosis, cysteine and methionine metabolism, hippo signaling pathway, proximal tube bicarbonate reclamation, and ribosome, respectively