Fig. 6

Differentially expressed gene analysis (DEG) and indicated gene verification in SLGCs. A The significantly upregulated (52) and downregulated (49) DEGs induced by Ad-BMP9 in iSLGCs. B KEGG enrichment signaling pathways included: amoebiasis (1), small-cell lung cancer (2), protein digestion and absorption (3), PPAR signaling pathway (4), and thiamine metabolism (5). C Overlap of metabolic gene expression between the cell line and tissues. There were 520 metabolism-related genes coexpressed between SLG and iSLGCs. D The expression of Pparγ-2 was increased upon the induction of Ad-BMP9. E The upregulated expression levels of Id1 and Smad8 were confirmed using RT-qPCR demonstrating the activity of BMP9. BMP9 stimulated Ctgf and Snai1 expression in iSLGCs. All experiments were repeated at least three times. **P < 0.01, ***P < 0.001