Fig. 3
From: Tight association of autophagy and cell cycle in leukemia cells
Autophagy inhibition enhances palbociclib-induced G1-phase arrest. Cells were exposed to palbociclib with or without autophagy inhibitors for 48 h. Autophagy and cell cycle phases were determined by flow-cytometric analysis of Cyto-ID + DRAQ5 double-stained cells. A Effect of palbociclib on cell cycle distribution. B, C Effect of palbociclib on autophagy. Cyto-ID fluorescence intensities were normalized to the mean Cyto-ID fluorescence intensities of untreated cells. Mean ± SEM of three independent measurements is shown (A: 3-MA or CQ versus control, C: G1 cells versus G2/M cells: *P < 0.05)