Fig. 4

Cell cycle phase and autophagy are tightly associated. Cells were exposed to 0.2 µM etoposide for the indicated times. Autophagy and cell cycle phases were determined by flow-cytometric analysis of Cyto-ID + DRAQ5 double-stained cells. A Effect of etoposide on cell cycle phase distribution and autophagy. B, C Cell populations were grouped into quintiles based on their Cyto-ID fluorescence intensities (Fig. S3A). B Relative Cyto-ID fluorescence intensities of the quintiles of cells. Cyto-ID fluorescence intensities were normalized to the mean Cyto-ID fluorescence intensity of untreated cells. C Cell cycle phase distribution in the quintiles of cells. Mean ± SEM of two (12-h and 16-h treatment) or three independent measurements is shown