Fig. 6
From: Tight association of autophagy and cell cycle in leukemia cells
Autophagic and metabolic activities are associated. Cells were flow-cytometrically sorted on the basis of their Cyto-ID fluorescence intensity into subpopulations with low, medium, and high Cyto-ID fluorescence (AutLO, AutME, and AutHI, respectively). The subpopulations were subjected to metabolic analysis immediately after sorting. A OCR as a proxy for oxidative metabolism was measured with a Seahorse XFe96 Analyzer using a Cell Mito Stress Test. ATP production was calculated from OCR data (see Fig. S6 for details). B ECAR was measured as a proxy for glycolytic activity. OCR and ECAR of the three fractions were normalized to OCR and ECAR of AutME. C Energetic maps of sorted cells generated from OCR and ECAR data presented in A and B, respectively. Means ± SEM of each three independent measurements are shown; each biological replicate consisted of six readings (*P < 0.05)