Fig. 7
From: Tight association of autophagy and cell cycle in leukemia cells
Cells with high autophagy are preferentially in the G2/M phase of the cell cycle. Cells were flow-cytometrically sorted on the basis of their Cyto-ID fluorescence intensity into subpopulations with low, medium, and high Cyto-ID fluorescence (AutLO, AutME, and AutHI, respectively). A, B Cells were fixed in ethanol approximately 1 h after sorting. Cell cycle phases were determined by flow-cytometric analysis of PI-stained ethanol-fixed cells. A Representative histograms of cell populations. B Quantification of cell cycle phases. C RNA was prepared approximately 1 h after sorting. mRNA expression levels were determined by real-time RT-PCR and normalized to B2M expression levels. Mean ± SEM of three independent measurements is shown (*P < 0.05). D Representative immunoblots of lysates from sorted cells. Lysates were prepared after 1-h cultivation of sorted cells in the absence or presence of 10 µM CQ. The loading controls are the same as in Fig. 5A since LC3B, cyclin B1, pS10-H3, and β-actin were detected on the same blots