Fig. 3

RNF7 knockdown inhibits tumor growth in vivo. Caki-1 cells were stably transduced with RNF7 shRNA or control scramble shRNA (shNC). Then, the cells were subcutaneously injected into nude mice (n = 6 per group). A Tumor volume was evaluated every 3 days for 33 days. B On day 33, the mice were sacrificed, and the tumors were photographed and weighed. C Representative images of TUNEL staining in xenograft mouse tumors. TUNEL-positive cells were quantified accordingly. Scale bar: 50 µm. D The protein levels of RNF7, STAT3, and p-STAT3 in xenograft mouse tumors were determined by Western blot. Experiments performed in triplicate; data expressed as mean ± SD (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 compared with shNC